|
14 Threepond
Road Smithtown, New York 11787 USA 888-ORAFLOW (USA) or 631-961-8875
(Int'l) Fax: 516-596-8788 |
|
|
|
|
| |
How Do I Calibrate Periotron
Professional For GCF? |
|
| |
Calibration Curve Periotron with PerioCol Collection Strips
Preparation of a standard curve relating Periotron readings to volume of
fluid in microliters.
Insert a blank PerioCol strip between the Periotron fluid meter sensors, and
set the instrument to read zero (0) by turning the dial to the left or
right. Discard the blank strip once set to zero.
Using a microliter syringe, which can accurately deliver 0.25 to 1.25 µl of
fluid, Periotron Scores are related to volume (µl) as follows: The syringe
is filled to capacity (1.25 - 5.0 µl, depending on the model of syringe
selected) with a test fluid making sure that the loaded syringe contains no
air bubbles. It makes little difference whether the test fluid is distilled
water, saliva or serum; the results are essentially the same, except for
water at the upper limits of the range. The plunger is carefully depressed
to deliver 0.25 µl, which appears as a minute droplet at the end of the
syringe tip. Without delay, touch the end of a PerioCol strip to the test
fluid droplet. Notice that it is immediately absorbed onto the strip. Again
without delay, place the strip between the Periotron sensors. A Periotron
Score is displayed shortly. Repeat this step three (3) more times with this
volume (0.25 µl) and record the average score.
Repeat the above step using volumes of 0.5 ul, 0.75 ul, 1.0 ul and 1.25 ul,
and in each case record the average Periotron score.
Once scores have been obtained for all volumes, Periotron Professional will
compute a standard curve relating the volume of fluid (µl) on the "x" axis
to the Periotron Score on the "y" axis. Please note that calibration usually
only needs to be set once. To check that the calibration has not or hardly
changed with time, simply measure the volumes of two (or more) known
samples.
Placing a strip containing an unknown volume of fluid (e.g. gingival crevice
fluid; GCF) or periodontal pocket fluid; PPF) between the sensors, a
Periotron Score is automatically determined. The volume of fluid (µl) is
determined by interpolation from the standard calibration curve. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
