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14 Threepond
Road Smithtown, New York 11787 USA 888-ORAFLOW (USA) or 631-961-8875
(Int'l) Fax: 516-596-8788 |
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How Do I Calibrate Periotron
Professional For Sialo? |
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Calibration Curve Periotron with SialoPaper Collection Strips
Preparation of a standard curve relating Periotron readings to volume of
saliva in microliters and relating the volume obtained to saliva thickness
in micrometers when oral wetness is being determined.
Insert a blank SialoPaper strip between the Periotron fluid meter sensors,
and set the instrument to read zero (0) by turning the dial to the left or
right. Discard the blank strip once the zero is set.
Using a microliter syringe, which can accurately deliver 1.0 µl quantities
of fluid and less, Periotron Scores are related to fluid volume (µl) as
follows: The syringe is filled to capacity (1.0 - 5.0 µl, depending on the
model of syringe selected) with a test fluid making sure that the loaded
syringe contains no air bubbles. The test fluid can be distilled water,
saliva or serum; the results are the same except for a small difference with
water at the upper limits of the range. The plunger is carefully depressed
to deliver 0.35 µl, which appears as a minute droplet at the end of the
syringe tip. Without delay, the flat surface of the SialoPaper strip is
carefully brought into contact with the droplet of test fluid. This
procedure is repeated rapidly with additional fluid from the syringe until a
total of 1 µl of test fluid has been accurately delivered to three (3)
separate areas on the SialoPaper strip. The strip is then placed between the
sensors of the Periotron 8000 meter so that the entire circular portion of
the SialoPaper covers the lower sensor of the Periotron instrument. The
Periotron Score on the digital readout is then recorded. This procedure is
repeated three (3) more times with this total volume (1µl) and the average
score is recorded.
Repeat the above step using volumes of 0.5, 1.5, 2.0, 2.5 and 3.0 ul, and
record the average Periotron score for each volume.
Once scores have been obtained for all volumes, enter them into Periotron
Professional which will compute a standard curve relating the volume of
fluid (µl) on the "x" axis to the Periotron Score on the "y" axis. Please
note that it is usually only necessary to do the calibration once. To check
that the calibration has not or has hardly changed with time, simply measure
the volumes of two (or more) known samples.
A strip containing an unknown volume of saliva is sampled from the mouth,
either from one or more minor salivary glands, duct openings of a major
salivary gland, or the residual saliva on an oral soft or hard tissue
surface. It is then placed between the sensors. The Periotron Score is
recorded and the volume of fluid is determined by interpolation from the
standard curve. Values obtained are converted to volumes in µl and for
residual saliva film, its value is converted to thickness in µm by Periotron
Professional.
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